Figure 1. PT2385 ablates ventilatory acclimatization to hypoxia in WT male mice.

(A) Graphs show changes in minute ventilation in response to an acute (5 minutes) challenge with 10% O₂/3% CO₂ (white bars) in male mice before (baseline) and following twice daily treatment with 10 mg/kg PT2385 (or vehicle), beginning 24 hours before 7-day exposure to hypoxia (H, 10% oxygen) and continuing throughout (to a total of 8 days of treatment) (n = 6). (B) Graph shows AVRs to challenges with 10% O₂/3% CO₂, quantified from the minute ventilation shown in A. Data were analyzed by 2-way repeated measures ANOVA with baseline recordings removed from the statistical analysis (P values in Table 1), followed by Holm-Sidak’s multiple comparisons 2-tailed test for individual time point comparisons, for which significance is reported in the graph. ****P < 0.0001.