Figure 4. Catecholamines are relatively increased in Asxl2^ΔLysM BAT.

(A) Tyrosine hydroxylase mRNA expression by naive and IL-4– or palmitate-treated Asxl2^fl/fl and Asxl2^ΔLysM BMMs. Adrenal tissue served as positive control. (B) Norepinephrine (NE) content of BAT of HFD-fed control and Asxl2^ΔLysM mice. (C) Glycerol released from BAT explants derived from HFD-fed control and Asxl2^ΔLysM mice. (D) Maoa mRNA abundance in BAT stromal vascular fraction of chow- or HFD-fed control and Asxl2^ΔLysM mice. Data are presented as mean ± SD. *P < 0.05, as determined by unpaired t test (B) or 2-way ANOVA with Holm-Sidak’s post hoc analysis for multiple comparisons (C and D).