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. 2020 Mar 23;130(5):2252–2269. doi: 10.1172/JCI132139

Figure 4. Behavioral assays identify reduced activity level in EAE adults.

Figure 4

(A) Food consumption assay design. (B) EAE has no impact on average daily food consumption in adult males (n = 10 animals/group). (C and D) EAE (1% EtOH) adults have a reduced swimming speed relative to controls (P < 0.0001, Student’s unpaired 2-tailed t test). (C) Average of group speed over 3-second intervals. (D) Individual speed averaged over 30-second intervals. ****P < 0.0001. (E–G) Swimming speed, distance traveled, and duration performing turns in a 1-minute monitored locomotion assay in control and EAE (1% EtOH) adults. EAE cohorts have a normal activity level under ND conditions, but show reduced activity and increased turning behavior with HFHC diet challenge. (E) ***P = 0.0005 and (G) *P = 0.0227, 2-way ANOVA with Šidák’s multiple comparisons test. (H and I) EAE adults spend more time near the rear of the tank during a 5-minute laminar flow challenge in a modified Blazka-type swim chamber. *P = 0.0324, Student’s unpaired 2-tailed t test. Error bars show mean with SD. Sample numbers (n) noted under figure panels.