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(A) Representative multicolor immunofluorescence images of CD8+ (green) GZMA+ (purple) T cells (delineated by arrows) in an SSc skin biopsy. (B) Relative proportions of CD4+ and CD8+ T cells expressing GZMA (n = 10). (C) Absolute numbers of CD4+GZMA+ and CD8+GZMA+ T cells in skin biopsies of 10 patients with SSc. Data are presented as mean ± SEM. **P < 0.01 by Mann-Whitney U test.
