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. 2020 Apr 23;11:556. doi: 10.3389/fmicb.2020.00556

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Copyright © 2020 Feng, Liu, Wang, Wang, Zang, Liu, Song, Gu, Fan, Yang, Chen and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bioinformatics analysis of the YbhFSR transporter. (A) Phylogenetic tree of YbhF. MEGA7.0 software was used to construct the phylogenetic tree of YbhF. (B) Alignment of the amino acid sequence of the YbhF-ORF1 region and YbhF-ORF2 region with other ABC drug-resistance transporters. There are at least six highly conserved motifs, in the order of walker A, Q-loop, ABC signature motif, walker B, D-loop and switch motif. TMHMM 2.0 software was used to predict transmembrane domain of YbhF (C), YbhS (D), and YbhR (E). YbhF protein encoded no transmembrane helical domain. YbhS and YbhR have six putative α-helical transmembrane segments.