FIGURE 8.

The subcellular localization of YbhF in E. coli/pET28a, E. coli/pET28a-ybhF, and E. coli Δ ybhF. Whole cells were subjected to immunogold labeling with antibody against YbhF. (A) E. coli/pET28a; only a small amount of scattered distribution was in the vicinity of the cell inner membrane. (B) E. coli/pET28a-ybhF; the YbhF protein is clustered in the vicinity of the cell inner membrane. (C) E. coli ΔybhF; only few colloidal gold particles in the cytoplasm and no one in the vicinity of the cell inner membrane.