TABLE 3.
Drug | Minimum inhibitory concentrations (μg/ml) |
||||||
E.coli/pET28a | E.coli/pET28a-ybhF | E.coli△ybhF | E.coli△ybhS | E.coli△ybhR | E.coli △acrB | E.coli△acrB△ybhF | |
Tetracycline | 1 | 2 | 0.5 | 1 | 0.5 | 0.25 | 0.125 |
Oxytetracycline | 32 | 128 | 16 | 32 | 16 | 8 | 4 |
Chlortetracycline | 64 | 64 | 1 | 64 | 1 | 0.125 | < 0.125 |
Doxycycline | 32 | 32 | 1 | 32 | 1 | 0.125 | < 0.125 |
Ethidium | 256 | 256 | 128 | 256 | 128 | 128 | 16 |
Hoechst | 128 | 128 | 64 | 128 | 64 | 64 | 32 |
Cefoperazone | 1 | 1 | 1 | 1 | 1 | 0.125 | 0.125 |
Cefazolin | 64 | 64 | 32 | 64 | 64 | 32 | 32 |
Streptomycin | > 256 | >256 | > 256 | >256 | > 256 | >256 | > 256 |
Ampicillin | 8 | 8 | 8 | 8 | 8 | 4 | 4 |
Roxithromycin | > 256 | >256 | > 256 | >256 | > 256 | >256 | > 256 |
Chloramphenicol | 32 | 32 | 32 | 32 | 32 | 0.5 | 0.5 |
Rifampicin | 16 | 16 | 16 | 16 | 16 | 8 | 8 |
Norfloxacin | 2 | 2 | 2 | 2 | 2 | < 0.125 | <0.125 |
Deoxycholate | > 256 | >256 | > 256 | >256 | > 256 | >256 | > 256 |
Sodium cholate | > 256 | >256 | > 256 | >256 | > 256 | >256 | > 256 |
Ofloxacin | 0.125 | 0.125 | 0.125 | 0.125 | 0.125 | < 0.125 | <0.125 |
Doxorubicin | 4 | 4 | 4 | 4 | 4 | 4 | 1 |
Daunorubicin | 64 | 64 | 64 | 64 | 64 | 1 | 1 |
Acridine flavin | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
Quinine | > 256 | >256 | > 256 | >256 | > 256 | 0.5 | 0.5 |