Figure 3. Proteins identified in utricle exosomes.

Proteins associated with exosomes and non-exosomal proteins secreted from heat-shocked utricles were identified using tandem mass spectrometry. Exosomes were isolated from conditioned media via size-exclusion chromatography and analyzed in parallel with the exosome-depleted media (non-exosomal fraction). (A) A total of 291 unique protein families were identified in exosomes, 56 of which were also found in the non-exosomal fraction. (B) The 20 most significantly enriched GO cellular component terms for proteins identified in utricle-derived exosomes. (C) The 20 most significantly enriched biological process GO terms for proteins identified in exosomes from utricles.