(A) Spontaneous hemolysis, (B) methemoglobin, (C) lactate, (D) pH, (E) glucose, and (F) sodium were measured in aliquots obtained from each unit at the designated storage time. G6PD-normal (blue; n = 27) and G6PD-deficient (red; n = 10) are represented. Mean and SD are shown. Statistical significance was calculated using 2-way repeated-measures ANOVA with Sidak’s multiple comparisons test; *P < 0.05. Thick dotted red line denotes the FDA criterion for the allowable spontaneous hemolysis rate during storage.