Fig. 9. Enhanced cell death in BCSCs treated with shRNA-C under oxidative stress conditions.

(A) Transfection efficiency images of shRNA-C expressing a lentiviral vector for the knock-down of the CD66c gene. (B) Cell viability of BCSCs treated with shRNA-C under oxidative stress conditions. Data are expressed as the mean ± SEM. **P < 0.02 for in the absence or presence of H₂O₂. ***P < 0.01 for H₂O₂-only group versus H₂O₂treated with shRNA-C group. (C) FACS analysis of cell death induced by treatment with H₂O₂ post-shRNA-C transfection in BCSCs. (D) Quantitative viable cell population of BCSCs treated with shRNA-C and H₂O₂ by FACS analysis. Data are expressed as the mean ± SEM. *P < 0.05 or **P < 0.02 for the comparison of no H₂O₂ treated groups and H₂O₂ treated groups or **P < 0.02 for shRNA-SC versus shRNA-C groups in the presence of H₂O₂. ***P < 0.01 for the comparison of NC and shRNA-C groups in the presence of H₂O₂. (E) Quantitative death cell population induced by shRNA-C treatment with H₂O₂. Data are expressed as the mean ± SEM. *P < 0.05 or **P < 0.02 for in the absence or presence of H₂O₂. **P < 0.02 for the comparison of shRNA-SC with H₂O₂ group and shRNA-C with H₂O₂ group. ***P < 0.01 for the comparison of H₂O₂ only group and shRNA-C with H₂O₂ group.