Figure 4.

Effects of miR-216a on the proliferation, migration, and invasion of PC cells. (A) The effects of miR-216a on PANC-1 cell proliferation were determined using MTT assays. (B) PANC-1 cells stably transcribing miR-216a showed decreased colony formation rates compared to control cells. (C) Wound healing and (D) Transwell assays in PANC-1 cells transfected with the miR-216a overexpression vector or negative control vector. (E) Increased expression of miR-216a reduced the invasive potency of TSPAN1-OV PANC-1 cells. Data are shown as the means ± S.D., n=3. *P<0.05, **P<0.01 was considered significant.