Figure 4.

CDKI-73 combined with olaparib synergistically induces cell apoptosis. A, B. Apoptosis was detected by flow cytometry. HO8910 and OVCAR-5 cells were exposed to CDKI-73 (0.25 μM) and olaparib (0.50 μM) or their combination for 48 hours, and apoptosis (annexin V+/PI- and annexin V+/PI+) was analyzed by flow cytometry. DMSO was used as a vehicle. Values are shown as the mean ± S.D. of three independent experiments. **P < 0.01; ***P < 0.001 (Student’s t-test). C. Western blot analysis of Bcl-2 and cleaved PARP in the two ovarian cancer cell lines treated with monotherapy or the combination of CDKI-73 and olaparib. Tubulin was used as a loading control.