Skip to main content
. 2020 Apr 7;20:621–638. doi: 10.1016/j.omtn.2020.03.013

Figure 2.

Figure 2

ZFAS1 Enhances OC Cell Proliferation, Migration, and Invasion by Suppressing miR-548e Expression

(A and B) The proliferation rates of SKOV3 and Caov3 cells transfected with shZFAS1 and miR-548e inhibitor. Cell proliferations were analyzed by CCK-8 (A) and clone formation assay (B), respectively. (C) The migration capacities of SKOV3 and Caov3 cells transfected with shZFAS1 and miR-548e inhibitor. Cell migration was analyzed by wound-healing assay. (D) The invasion capacities of SKOV3 and Caov3 cells transfected with shZFAS1 and miR-548e inhibitor. Cell invasion capacity was assessed using the Transwell system. (E) Protein abundances of E-cadherin, N-cadherin, vimentin, MMP-2, and Slug in SKOV3 and Caov3 cells transfected with shZFAS1 and miR-548e inhibitor. Protein levels were determined by western blotting. ZFAS1, zinc finger antisense 1; shZFAS1, short hairpin RNA targeting ZFAS1; NC, negative control; MMP-2, matrix metalloproteinases 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.