Figure 8.

ZFAS1 Promotes OC Cisplatin Resistance by Suppressing the let-7a/BCL-XL/S Signaling Axis

(A) ZFAS1 relative expression levels in SKOV3 and Caov3 cells with ZFAS1 overexpression and transfected with let-7a mimics. ZFAS1 expression was detected by the qRT-PCR method. (B) Relative let-7a expression levels in SKOV3 and Caov3 cells with ZFAS1 overexpression and transfected with let-7a mimics by the qRT-PCR method. (C) BCL-XL/S protein abundances in SKOV3 and Caov3 cells with elevated ZFAS1 and let-7a expression by western blotting. GAPDH was included as the internal standard. (D) Cisplatin resistances of SKOV3 and Caov3 cells with ZFAS1 overexpression and transfected with let-7a mimics. Cell viabilities measured by the CCK-8 method under cisplatin treatments were used to calculate the IC₅₀ values. (E) The apoptosis of SKOV3 and Caov3 cells with elevated ZFAS1 and let-7a expression by flow cytometry. (F) Tumors generated from SKOV3 cells transfected let-7a mimics in nude mice under cisplatin treatment. Cell line-based xenograft (CDX) models were established using nude mice to evaluated cisplatin resistance in vivo. (G) The sizes and weights of tumors developed from SKOV3 cells transfected let-7a mimics in nude mice under cisplatin treatment. (H) Ki-67 protein levels in tumors generated from SKOV3 cells transfected let-7a mimics in nude mice under cisplatin treatment. Scale bar: 50 μm. (I) BCL-XL/S mRNA levels in tumors developed from SKOV3 cells transfected let-7a mimics in nude mice under cisplatin treatment. ZFAS1, Zinc finger antisense 1; NC, negative control; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IC₅₀, half maximal inhibitory concentration; ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.