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ARNTL2 knockdown inhibited the proliferation of HCT-15 and HCT-116 cells. A, B. In the CCK-8 assay, both HCT-15 and HCT-116 cells transfected with si-ARNTL2 (si-1, si-2) showed a lower level of cellular proliferation activity, compared with the negative control (*P<0.05). C-E. Colony formation study showed that both HCT-15 and HCT-116 cells transfected with si-ARNTL2 (si-1, si-2) had a 3-fold decrease in colony formation rate, compared with the NC group (*P<0.05).
