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The four analyzed CpG sites in the angiotensinogen (AGT) promoter region. The four CpG sites analyzed by pyrosequencing were situated 282–229 base pairs (bp) upstream of the transcription start site of AGT in a CEBP binding region (CpG 1: −282; CpG 2: −261; CpG 3: −245; CpG 4: −229). Lower DNA methylation of these sites has been associated with a higher transcriptional activity of AGT (Wang et al., 2014).
