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. 2020 Feb 20;14(5):1074–1088. doi: 10.1002/1878-0261.12631

Figure 1.

Figure 1

ZFPM2‐AS1 was upregulated in LUAD, promoting proliferation in LUAD cells. (A) Upregulation of ZFPM2‐AS1 in LUAD samples was obtained in GEPIA database. (B) RT‐qPCR results of the upregulation of ZFPM2‐AS1 in LUAD cell lines (mean ± SD; n = 6; one‐way ANOVA). (C) RT‐qPCR results of the overexpression of ZFPM2‐AS1 by pcDNA3.1/ZFPM2‐AS1 in A549 cells and the knockdown of ZFPM2‐AS1 by si‐ZFPM2‐AS1#1/2/3 in SPC‐A1 cells (mean ± SD; n = 6; Student’s t‐test and one‐way ANOVA). (D, E) CCK‐8 (mean ± SD; n = 6; two‐way ANOVA) and EdU assays (scale bar = 100 μm; mean ± SD; n = 6; Student’s t‐test and one‐way ANOVA) were used to assess proliferation of LUAD cells upon ZFPM2‐AS1 overexpression and knockdown. *P < 0.05, **P < 0.01, ***P < 0.001.