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. 2020 Feb 20;14(5):1074–1088. doi: 10.1002/1878-0261.12631

Figure 4.

Figure 4

ZFPM2‐AS1 potentially regulated ZFPM2 through interacting with UPF1. (A, B) FISH (scale bar = 10 μm; n = 3) and subcellular fractionation assays were used to determine the cellular localization of ZFPM2‐AS1 in LUAD cells. (C) Luciferase reporter assay (mean ± SD; n = 6; Student’s t‐test and one‐way ANOVA) was used to assess the effect of ZFPM2‐AS1 on promoter transcription of ZFPM2. (D) Pull‐down assay followed by MS was used to identify the interacting partner for ZFPM2‐AS1. (E, F) RIP (mean ± SD; n = 6; Student’s t‐test) and western blot assay after pull‐down assays confirmed the interaction between ZFPM2‐AS1 and UPF1. (G) FISH assay (scale bar = 10 μm; n = 3) confirmed the overlapped expression of ZFPM2‐AS1 in cytoplasm of LUAD cells. ***P < 0.001, n.s: no significance.