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. 2020 Mar 16;14(5):1045–1058. doi: 10.1002/1878-0261.12652

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© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Matrix Gla protein interacted with p‐STAT5 in GC cells. (A) GESA analysis indicated that the expression level of MGP was positively correlated with both hallmark of apoptosis (left) and hallmark of IL2/Stat5 signaling (right). (B) Cellular immunofluorescence colocalization detected by confocal microscopy in BGC823 (upper panel) and AGS (lower panel). MGP (green) and STAT5 (red) pefectly colocalized with each other. DAPI was used to stain nuclei, and magnified 3D projection was displayed in the right panel. (C, D) Co‐IP assays were performed in BGC823 and AGS cells with both MGP (two above) and STAT5 (two below) antibodies, which indicated a strong interaction between MGP and STAT5. Three samples were pipetted into three different lanes according to the antibodies added or not added to the samples (input group: no antibody; IgG group: IgG; MGP group: anti‐MGP antibody; STAT5 group: anti‐STAT5 antibody). The protein levels of MGP and STAT5 were detected by western blot analysis.