Table 1.

Comparison of VAF of all detected mutations in ctDNA isolated from blood collected in EDTA or Streck tubes.

Pat	Mutation	Nucleotide substitution	VAF %	VAF %
			EDTA	Streck
MM01	CDKN2A R80X	NM_000077.4:c.238C>T	0.3	0.3
	KIT V559A	NM_000222.2:c.1676T>C	0.9	1.1
MM10	BRAF K601E	NM_004333.6:c.1801A>G	0.4	0.5
	DPH3 ‐8C>T	NC_000003.12:g.16306504T>C	1.2	1.3
	RPS27 UTR MUT	NC_000001.11:g.153990763C>T	1.4	1.4
MM13	BRAF K601E	NM_004333.6:c.1801A>G	0.5	WT
	MAP2K1 I111S	NM_002755.3:c.332T>G	WT	0.3
MM14	NRAS Q61R	NM_002524.5:c.182A>G	2.4	2.6
	MAP2K1 N382H	NM_006049.4:c.*162T>G	0.4	0.5
MM15	MAP2K1 I111S	NM_002755.3:c.332T>G	0.5	0.4
	MAP2K1 N382H	NM_006049.4:c.*162T>G	0.6	0.6
	KIT V559A	NM_000222.2:c.1676T>C	0.2	0.3
MM17	MAP2K1 I111S	NM_002755.3:c.332T>G	0.4	0.6
	MAP2K1 N382H	NM_006049.4:c.*162T>G	0.7	0.6
	NRAS Q61R	NM_002524.5:c.182A>G	1.4	1.4
MM18	CDKN2A R80X	NM_000077.4:c.238C>T	0.3	0.2
	MAP2K1 N382H	NM_006049.4:c.*162T>G	0.5	0.6
MM19	MAP2K1 I111S	NM_002755.3:c.332T>G	0.5	0.4
	MAP2K1 N382H	NM_006049.4:c.*162T>G	0.5	0.4