TABLE 2.

API sugar utilization test.

Substrate/Assay	CBS 707	KBI 1.2	KBI 3.2	KBI 5.3	KBI 12.1
Control	−	−	−	−	−
D-Galactose	+	+	+	+	+
Cycloheximide (Actidione)	+	+	+	+	+
D-Saccharose	+	+	+	+	+
Lactic acid	−1	w	w	−	w
D-Cellobiose	+	w	w	−	+
D-Raffinose	+	+	+	+	+
D-Maltose	+	+	+	+	+
D-Trehalose	+	+	+	+	+
Potassium 2-Ketogluconate	w	w	w	w	w
Methyl-αD-Glucopyranoside	+	+	+	+	+
D-Mannitol	+	+	+	w	+
D-Sorbitol	+	+	+	+	+
Palatinose	+	+	+	+	+
D-Melezitose	w	+	w	w	+
Potassium gluconate	w	−	w	−	w
D-Glucose	+	+	+	+	+
L-Sorbose	w	+	w	+	+
Esculin ferric citrate	w	+	+	w	+
Flocculation (%)	15 ± 2a	88 ± 10b	28 ± 1a	25 ± 8a	20 ± 6a
Definition	Non-flocculent	Very flocculent	Moderately flocculent	Moderately flocculent	Moderately flocculent
Phenolic off-flavor	Negative	Negative	Negative	Negative	Negative

− negative; + positive; w weak. Substrates negative for all strains are not included in the table. Different letters in superscripts indicate a significant difference of the means within a row (p ≤ 0.05). The full table of substrates is included in Supplementary Data Sheet S1. ¹Deviation from literature which states a positive reaction (Kurtzman et al., 2011).