Table 1. Sequence of primers of Hif1, VEGFR1, VEGFR2 genes and GAPDH .

Gene	Sequence of primer	Length of product	Tm (°C)
VEGFR1	Forward:5ʹ GCACATGACGGAAGGAAGAC 3ʹ	187	60
	Reverse:5ʹ TTCGCAGTTCAGCAGTCCTA 3ʹ
VEGFR2	Forward:5ʹ ACGAGGAGAGAGGGTCATCT 3ʹ	93	60
	Reverse1:5ʹ GACACACTCTCCTGCTCAGT 3ʹ
GAPDH	Forward:5ʹ TCGGAGTCAACGGATTTG 3ʹ	219	60
	Reverse:5ʹ CCTGGAAGATGGTGATGG 3ʹ
HIF1α	Forward:5ʹ CCAACCTCAGTGTGGGTACA 3ʹ	254	60
	Reverse:5ʹ CGGCTCATAACCCATCAACT 3ʹ