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. 2020 Feb 18;10(2):307–314. doi: 10.34172/apb.2020.037

Figure 2.

Figure 2

Morphological features of bone marrow derived-MSCs; (A) Spindle-shaped morphology of bone marrow cells at day 7, (B) More confluent bone marrow derived-MSCs at 21 days of culturing cells. Multi-lineage differentiation of bone marrow derived-MSCs;(C)Generation of lipid vacuoles after adipogenesis and staining by Sudan III; (D) Osteogenic differentiation and Alizarin red staining of mineralized cell aggregates; (E) Chondrogenic differentiation and Toluidine blue staining. (F) The expression of the cell surface markers of bone marrow derived-MSCs that analyzed by flow cytometry. Each antibody was tested individually and the isotopes controls were used as the negative control in this experiment; (A) a total population of cells, the bone marrow derived-MSCs were positive for CD73 (95.5%) and CD44 (90.2%) and negative for CD31 (3.02%) and CD34 (6.3 %). For CD31 and CD34, the isotype control was mouse IgG1 and for CD73 and CD44, the isotype control was mouse IgG2b. Also, isotype control is seen with red dots.