Fig. 3.

(Left): IR-NIPI temperature log of 96-well plate loaded with 50 μL aliquots of CPA where half of the wells were nucleated manually with ice mist (green lines) and half left to nucleate without control (blue lines). The data gap at around 1300 s is due to the apparatus being disabled to allow the manual nucleation procedure. Right: IR colour map mages of the plate undergoing cooling at progressing time points – manually nucleated wells are on the right half of the plate, uncontrolled on the left. Recently frozen wells having released latent heat appear yellow (temperature-colour scale below is approximate). A plastic film covering the left side of the plate appears as an orange region in the image taken at 1000s. This was placed to protect the wells on this side of the plate from the nucleation inducing ice mist. After manual nucleation was done the film was removed so is no longer visible by the next image at 1500 s. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)