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. 2020 Apr 23;11:381. doi: 10.3389/fphys.2020.00381

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Copyright © 2020 Xie, Huang, Ling, Qin, Wang and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of chemerin and CMKLR1 in diabetic cardiomyopathy (DCM) rats. (A) The serum levels of chemerin in the control and DCM groups. (B) Transfection efficiency of CMKLR1-siRNA in myocardium. Bright green points (white arrow) indicate GFP with lentivirus-CMKLR1-siRNA (scale bar: 20 μm). Relative mRNA (C–D) and protein (E–G) expression of chemerin and CMKLR1 in cardiac tissue with CMKLR1-siRNA treatment. Immunohistochemical staining (H) of chemerin and CMKLR1 in the different groups; scale bar: 20 μm. Data were presented as means ± SEM, n = 4–6. *p < 0.05, **p < 0.01 vs. Ctrl; ^§§ p < 0.01 vs. DCM+NC. NC, negative control.