Figure 5.

Loss of Wnt signaling attenuates inflammatory gene expression in response to classical polarizing stimuli. Macrophageal Wnt secretion was inhibited A) pharmacologically using Wnt-C59 or B) genetically by treating Csf1riCre+; Wls^fl/fl mice with tamoxifen prior to culture of macrophages. Wls expression was used to verify pharmacological inhibition of Wnt secretion, as evidenced by its upregulation upon treatment with Wnt-C59, as well as genetic knockout of Wls, as evidenced by nearly absent expression. Pro-(Il1b, Tnf, Nos2) or anti-inflammatory (Il10, Arg1) genes in response to M1 (lipopolysaccharide) or M2 (Interleukin-4 and −13) stimuli. p<0.05: # vs. M0, $ vs. M1, % vs. M2, A vs. Control of corresponding group.