Skip to main content
. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Biomaterials. 2020 Feb 27;243:119920. doi: 10.1016/j.biomaterials.2020.119920

Figure 9.

Figure 9.

Recombinant Wnt ligands rescue impaired Wnt-inhibited macrophage polarization. Macrophages were treated with 500nM Wnt-C59 or equivalent volume of DMSO vehicle and plated on smooth or rough-hydro surfaces. After 2 hours, macrophages were (A) left untreated to observe only surface effects, treated with (B) Wnt3a, −5a, −7a, or −9b on smooth surfaces, or (C) Wnt5b, −7a, −9b, or −11 on rough-hydro surfaces for 24 hours. Pro-(IL-1β, IL-6, IL12(p40), CXCL-10, TNF-α) and anti-inflammatory (IL-4, IL-10) chemokines and cytokines were measured by ELISA and normalized to DNA quantity in each sample (n=6 per group). p<0.05: # vs. TCPS-Vehicle, A vs. vehicle of corresponding vehicle group, B vs. control corresponding Wnt-C59-treated group, C vs. Wnt-C59 + Wnt3a group, D vs. Wnt-C59 + Wnt5a group, E vs. Wnt-C59 + Wnt7a group, F vs. Wnt-C59 + Wnt5b group, G vs. Wnt-C59 + Wnt11 group.