Fig. 1.

Treatment with pasteurized Akkermansia muciniphila reduces fat mass. Outline of study design (A). Twelve-week-old female mice were treated with either vehicle (Veh) or pasteurized Akkermansia muciniphila (pAkk) by daily oral gavage during 28 days, starting 3 days before ovariectomy (Ovx) or sham surgery. At the end of the study, body weight (B) and percentage of retroperitoneal fat (C) were measured. Values are given as means ± SE (n = 8–12). The overall effect of treatment (Veh/pAkk) and surgical procedure (Sham/Ovx) and their interaction were calculated using two-way ANOVA followed by Tukey’s post hoc test to correct for multiple comparisons between all groups, *P ≤ 0.05 for pAkk-treated sham mice and pAkk-treated ovx-mice vs. veh-treated ovx-mice.