Table 2.

Bone parameters in femur

Treatment	Surgery		Two-Way ANOVA (P Value)
	Sham	Ovx	Sham/Ovx	Veh/pAkk	Interaction
Tb N, 1/mm
Veh	3.26 ± 0.09	3.22 ± 0.15	NS	NS	NS
pAkk	3.07 ± 0.18	3.10 ± 0.10
Tb Sp, mm
Veh	0.128 ± 0.001	0.126 ± 0.001	NS	NS	NS
pAkk	0.128 ± 0.002	128.2 ± 0.001
Crt Thk, mm
Veh	0.197 ± 0.002	0.186 ± 0.003	P ≤ 0.01	NS	NS
pAkk	0.192 ± 0.002	0.184 ± 0.002
Femur length, mm
Veh	15.37 ± 0.15	15.61 ± 0.08	NS	P ≤ 0.05	NS
pAkk	15.19 ± 0.07	15.27 ± 0.13

Twelve-week-old female mice were treated with either vehicle (Veh) or pasteurized Akkermansia muciniphila (pAkk) by daily oral gavage during 28 days, starting 3 days before ovariectomy (Ovx) or sham surgery. At the end of the experiment, dissected femurs were analyzed with high-resolution μCT. Trabecular number (Tb N), trabecular separation (Tb Sp), cortical thickness (Crt Thk), and femur length were analyzed. Values are given as means ± SE, (n = 8–12). Two-way ANOVA to test the effect of treatment (Veh/pAkk) and surgical procedure (Sham/Ovx) and their interaction was used.