Fig. 2.

Effects of TNF-α on TTX-resistant (TTX-R) and voltage-dependent Na⁺ channel subtype Na_V1.8 (Na_V1.8) currents in rat dorsal root ganglion (DRG) neurons of control and occlusion groups. TTX-R currents were examined in DRG neurons after 24 h of exposure to TNF-α. A: representative current traces showing that TNF-α amplified TTX-R current in a dose-related manner in DRG neurons of both control limbs and occluded limbs. B: averaged data demonstrate that TNF-α amplified TTX-R currents and Na_V1.8 currents in DRG neurons of control limbs and occluded limbs. ○, Individual data points; n is the number of DRG neurons. $I_{{\text{Na}}_{\text{V}}1.8}$, peak current after TTX − peak currents after both TTX and A803467. In the control group, *P = 0.005 and P = 0.001 compared with TNF-α null after TTX and *P = 0.002 and P < 0.001 for $I_{{\text{Na}}_{\text{V}}1.8}$ current. In the occlusion group, *P = 0.041 and P = 0.020 compared with TNF-α null after TTX and *P = 0.047 and P = 0.021 for $I_{{\text{Na}}_{\text{V}}1.8}$ current.