Fig. 5.

cGMP generation and intracellular Ca²⁺ levels by atrial natriuretic peptide analog (MANP) in human primary aortic smooth muscle cells (HASMC) and vasorelaxation in dog arterial rings. A: intracellular cGMP levels by 0 [control (Ctrl)], 10⁻⁸, or 10⁻⁷ M MANP. *P < 0.05 vs. Ctrl, unpaired t test, n = 3. B: intracellular Ca²⁺ levels by 10⁻⁷ M angiotensin II (ANG II) in the absence or presence of 10⁻⁷ M MANP. Cells were preincubated with MANP for 5 min before ANG II stimulation. #P < 0.05 vs. ANG II, 2-way ANOVA followed by Bonferroni’s post hoc test, n = 8. C: ex vivo arterial relaxation by MANP in healthy dog femoral arterial rings. *P < 0.05 vs. baseline 100%, unpaired t test, n = 5.