Fig. 5.

Glutamine synthetase (GS) and electrogenic Na⁺-bicarbonate cotransporter 1 (NBCe1) expression. A: immunoblot analysis of GS with quantification in homogenates of the renal cortex of sham-operated control (SOC) mice, orchiectomized (ORCH) mice that received placebo pellets that released no hormone (ORCH + P), and mice that had a controlled-release pellet (60-day release) containing 1.5 mg/pellet of testosterone implanted subcutaneously at the time of ORCH (ORCH + T). There was no significant difference in GS protein expression between groups. n = 6 SOC mice, 6 ORCH + P mice, and 5 ORCH + T mice. B: immunoblot analysis of NBCe1 with quantification in homogenates of the renal cortex of SOC, ORCH + P, and ORCH + T mice. There was no significant difference in NBCe1 protein expression between groups. n = 6 SOC mice, 6 ORCH + P mice, and 5 ORCH + T mice. NS, not significant.