Fig. 1.

MicroRNA-214 (miR-214) is induced in both in vivo and in vitro models of renal ischemia-reperfusion. A: C57BL/6J male mice were subjected to 25 min of bilateral renal ischemia (I25) with 24 h (R24h), 48 h (R48h), 72 h, or 7 days (R7d) of reperfusion or were subjected to sham operation. miR-214 was quantified by quantitative PCR showing the induction of miR-214 in kidney tissues after ischemia followed by reperfusion of 48 h to 7 days (n = 6). B: rat kidney proximal tubular cells (RPTCs) were treated with 10 mM azide for 3 h for ATP depletion followed by 2 h of recovery in full culture medium (n = 3). miR-214 was quantified by quantitative PCR, showing the induction of miR-214 after ATP depletion in RPTCs. C: representative images and quantitative analysis of apoptotic cells. The percentage of apoptotic cells was determined by evaluating the cells with typical apoptotic morphology. D: representative immunoblots and densitometry analysis of cleaved caspase 3 in RPTCs. β-actin was used as a loading control. Con, control; ns, not significant. *P < 0.05; **P < 0.01; ****P < 0.0001.