Fig. 3.

MicroRNA-214 (miR-214) increases ATP depletion-induced apoptosis in rat kidney proximal tubular cells (RPTCs). RPTCs were transfected with empty vector or miR-214 plasmid before being subjected to ATP depletion (n = 3). A: representative images and quantitative analysis of apoptotic cells showing miR-214 overexpression increased apoptosis following ATP depletion in RPTCs. B: representative immunoblots and densitometry analysis of cleaved caspase 3 showing miR-214 increased active caspase 3 following ATP depletion in RPTCs. β-Actin was used as a loading control. I25R48h, 25 min of bilateral renal ischemia followed by 48 h of reperfusion. *P < 0.05.