Fig. 2.

Canagliflozin (CANA) attenuates cisplatin-induced apoptosis in rat kidney proximal tubular cells (RPTCs). RPTCs were treated with 20 μM cisplatin in the absence or presence of CANA. A: immunoblots of Na⁺-glucose cotransporter 2 (SGLT2) in RPTCs and kidney, liver, spleen, and heart tissues. B: immunoblots of SGLT2. RPTCs with or without CANA were treated with 20 μM cisplatin or vehicle for 16 h. Cyclophilin B was used as an internal loading control. C: representative images of cell morphology and nuclear morphology by Hoechst 33342 staining (scale bar = 0.4 mm). D: percentage of apoptosis (n = 6). E: caspase activity. Data are expressed as means ± SD; n = 3. F: immunoblots of cleaved caspase-3 and poly(ADP-ribose) polymerase-1 (PARP-1). β-Actin was used as the internal loading control. Immunoblots are representative of at least 3 independent experiments. *P < 0.05 vs. control groups; #P < 0.05 vs. the cisplatin-treated group without CANA.