Fig. 7.

LY294002 antagonizes the protective effect of canagliflozin (CANA) on cisplatin-induced apoptosis in rat kidney proximal tubular cells (RPTCs). RPTCs were treated with 20 μM cisplatin in the absence or presence of 15 μM CANA. LY294002 (50 μM) was used to inhibit Akt. A: representative immunoblots of phosphorylated Akt (Ser⁴⁷³) and total Akt with cyclophilin B as an internal loading control. B: percentage of apoptosis. *P < 0.05 vs. the control group; #P < 0.05 vs. the cisplatin only-treated group. C: representative images of cell and nuclear morphology after staining with Hoechst 33342. Scale bar = 0.2 mm. D: representative immunoblots of Bax and cytochrome c (Cyto c) in cytosol and mitochondrial fractions. Heat shock protein (HSP)60 and GAPDH were used as internal loading controls for mitochondrial and cytosol fractions, respectively.