Fig. 2.

Identification of transcription factors (TFs) regulating liver sinusoidal endothelial cell (LSEC) marker gene expression (A). Expression of LSEC-specific TFs in freshly isolated (t = 0 h) primary mouse LSECs (closed bars), primary mouse LSECs cultured for 12 h (open bars), or primary mouse LSECs cultured for 24 h (shaded bars). Data were analyzed by ANOVA and are expressed as mean ± SE and represent expression levels relative to t = 0 h (n = 3; *P < 0.05; *²P < 0.01; *³P < 0.001). LSEC signature genes induced after 6 days of lentiviral overexpression in human umbilical vein endothelial cells of Zinc finger E-box-binding protein 2 (ZEB2) (B), homeobox B5 (HOXB5) (C), Cut-like homolog 2 (Cux2) (D), transcription factor EC (TCFEC) (E), musculoaponeurotic fibrosarcoma (C-MAF) (F), MEIS homeobox 2 (MEIS2) (G), or GATA binding protein 4 (GATA4) (H). Genes with significant (*P < 0.05) induction, borderline significant (#0.05 < P < 0.1) induction or at least twofold not significant (P > 0.1) induction are indicated in closed squares, shaded triangles or lightly shaded diamonds, respectively. Insets: pie charts of corresponding distributions of these four categories of B–H. Data are expressed as means ± SE and represent fold increase relative to control virus (n = 6–8 from 2 independent experiments; Wilcoxon signed ranked test).