Table 1.
Primer sequences used for reverse transcriptase real-time polymerase chain reaction
| Gene Name | Forward Primer | Reverse Primer |
|---|---|---|
| HIF1a | GTTACGATTGTGAAGTTA | AAGGAATGAGATTAGGAA |
| Arnt1/HIF1b | GAGTGAGTGAGTGAGTGA | ATCTGTCTCTGTCTCTTACT |
| Epas1/HIF2a | TCATTCATATCCATACTAACAAG | CAACAAGAACAAGAACACT |
| HIF3a | TGCCACTACCACTACATT | CATACACAGAGACTCATACAC |
| Cldn1 | ATGCCTTCAACTGTTCTGTATCTC | AATCCAGGTCTACCAATGTCAATG |
| Cldn2 | AAGACCACAGCACCAGAG | CCACCTCAAGCACAATCC |
| Cldn3 | TGGAAGAGGACAGACAGG | ATTCGGCTTGGACAGTTC |
| Cldn4 | CGCTACTCTTGCCATTAC | CGTCCATCCACTCTACAG |
| Cldn5 | AGATGCTGCCTTAATGTCC | GGTTCAGTAGGTTCTTCTCC |
| Cldn7 | GCCTTAATGGTGGTGTCC | TCTTCGCTTTGTCATCTCC |
| Cldn8 | TCAGTATGTGTAGTTGTG | ATAATACCTCATTCTGCTAA |
| Cldn12 | TGGCTTCTTCATTGGTTTCAG | GACGCATTGTTAGTGTAATTGG |
| Cldn13 | GAGCCAACAACGATACCTTAG | TAGCCGCATCCAGAGTCC |
| Cldn15 | TATGCTGGAACCAAGTATG | GTATCACAACCGTAGAAGG |
| Jama | AAGCAGCAATTAGCAAGATAGG | ACAGCCGAGTTGGTTGAAG |
| Ocln | CTGCTGCTGATGAATATAATAG | CCTCTTGATGTGCGATAA |
| Tjp1 | CATAGTTCAACACAGCCTCCAG | CCATCCTCATCTTCATCTTCTTCC |
| Tjp2 | GCTGTGGACCTGCTCAACTAC | GGACTCAACCTCTGCCTTATGG |
| Tjp3 | CTCTTCACCGCCACCATCC | CGCCTGCTGTTGCTGTATTAC |
| Cgn | GCAAGAGGCTGAGAACAAG | CTTCATCTAACTCCGCTTCC |
| Marveld2 | TGTTGCTGCTGCTGCTATG | CCTTGCTCACTATGGACTGC |
| Cxadr | CCAAGACGCAGTATAACC | GGAATCATCACAGGAACC |
| Cdh1 | TTGTTCGGCTATGTGTCT | TGTGTACCTAAGAATCTGAGA |
| Cdh2 | ACAGAATATCAGATACACAA | AAGGAAGGTAGCATTATAG |
| Cdh3 | TATCACAACTCTGTCTCA | GGCAGGTTATCAATACTC |
| Cdh5 | CTCACAGACATAGAATAAGGT | TTACAGCAATGACTACAGAA |
| Ctnna1 | ACTTGATGAATGCTGTTGTG | TAATCTTGGTCTGCGTCTC |
| Ctnna2 | TCCTCCTGTGGTAGTTGC | CTGGTGTATGACGGTGTTC |
| Ctnna3 | TGACACAAGCAATGACATC | ATCTGGACACTGGTTAGC |
| Ctnnb1 | GGTCCTCTGTGAACTTGC | GTAATCCTGTGGCTTGTCC |
| Ctnnd1 | CTGCTCTGGTCCGATTGC | GTGGCTTACAGTCTTCATTAGG |
| Nectin | CTTATCTGTCAAGTGCTA | TCCGTCTCAATAATTCTG |
| Afdn | TATTCCTGTGATGTTGTC | CATAATCTGCTCCTTCAA |
| Jup | ACACTCAAGAAGACTACC | TTGTGGACATCTGGTATT |
| Ajap1 | CAACTTCCATCCGTCAGA | AACCACAGCATTCTTAATTCT |
Afdn, afadin/adherens junction formation factor; Ajap1, adherens junction-associated protein 1; Arnt, aryl hydrocarbon receptor nuclear translocator; Cdh, cadherin; Cgn; cingulin; Cldn, claudin family gene; Ctnna, catenin α; Ctnnb, catenin β; Ctnnd, catenin δ; Cxadr; coxsackie virus and adenovirus receptor; Epas, endothelial Per-Arnt-Sim domain-containing protein; HIF, hypoxia-inducible factor; Jama, junctional adhesion molecule A; Jup, junction plankoglobin; Marveld2, MARVEL (MAL and related proteins for vesicle trafficking and membrane link) domain-containing protein 2; Nectin, nectin cell adhesion molecule; Ocln, occludin; Tjp, tight junction protein.