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. 2020 Feb 24;318(4):G781–G792. doi: 10.1152/ajpgi.00195.2019

Fig. 5.

Fig. 5.

Relationship of actin cytoskeleton, Rho-associated protein kinase-1 (ROCK1), and p120 to E-cadherin in necrotizing enterocolitis (NEC). A: immunofluorescence micrographs of Caco-2 cells stained for E-cadherin (green, top) and actin (red, bottom) show matching expression in all groups; nuclei stained with DAPI (blue), ×100 scale bar, 20 µm. B: representative immunofluorescence micrographs of rat intestinal epithelium stained for ROCK1 (red, top), E-cadherin (green, middle), and composite (yellow, bottom). White arrows indicate areas of colocalization of E-cadherin and ROCK1; nuclei stained with DAPI (blue), ×500 scale bar, 5 µm. C: Western blot analysis showing coimmunoprecipitation of E-cadherin and ROCK1 with ROCK1 and p120 in all 4 experimental groups [control, ROCK1 inhibitor (RI; Y-27632), RI+NEC, NEC]. E-cadherin is associated with p120 but not with ROCK1. ROCK1 is not associated with E-cadherin or p120.