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. 2020 Feb 19;318(4):L723–L741. doi: 10.1152/ajplung.00255.2019

Fig. 5.

Fig. 5.

The effect of adipose tissue exosome (AT-Exo) on ventilator-induced lung injury (VILI) in vivo. A: hematoxylin and eosin (HE) staining (magnification, ×200 and ×400); lung injury scores are used for the semiquantitative analysis of lung histopathological damage. B: the wet/dry (W/D) weight ratio. C: protein concentrations in bronchoalveolar lavage fluid (BALF). D: myeloperoxidase (MPO) activity in lung tissue. E: proinflammatory cytokines TNF-α and IL-6 in BALF. F: quantitative real-time PCR (qRT-PCR) analysis shows the gene expression of TNF-α, IL-6, transient receptor potential vanilloid 4 (TRPV4), β-catenin, and VE-cadherin normalized to the gene expression of GAPDH. G: Western blot (WB) analysis shows the protein expression of TNF-α, IL-6, TRPV4, β-catenin, and VE-cadherin, and the relative values are normalized to GAPDH; n = 4 mice from each group assayed in triplicate. The results are expressed as means ± SE. *P < 0.05. CM, conditioned media; MV, mechanical ventilation; SB, spontaneously breathing.