Fig. 1.
Crtap expression and postnatal lung histology. A: Western blot analysis of wild-type (WT) whole lung lysates at different ages probed with a rabbit polyclonal antibody against CRTAP. A lysate of a 1-mo-old CrtapKO [knockout (KO)] lung was also included as the negative control. B: densitometry of the band products shown in A expressed as a ratio between Crtap and β-actin expression levels. NB, newborn; P5, 5 days old; P10, 10 days old; m, age in months. Results are representative from n = 1 sample/age and three technical replicates. C: Western blot showing CRTAP expression in adult human lung tissue. D, E, and G: histological sections of WT and CrtapKO lungs collected at P5, 3 mo, and 8–9 mo of age stained with hematoxylin and eosin (H&E) or Masson’s. F: quantification of the lung parenchyma defect at 3 mo and at 7–11 mo of age (n = 5 or 6 and n = 4 or 5 mice/genotype, respectively; 10 fields analyzed/mouse at ×20 magnification; Student’s t test) using the mean linear intercept (Lm) method.