Fig. 1.

Dose response of mouse recombinant (r)IFN-γ induces an upregulation of PD-L1 expression on CRL-2167 (CRL) cells. Cells were plated and stimulated with mouse rIFN-γ in a dose-response manner to determine the optimum concentration for the expression of PD-L1 determined by flow cytometry. PD-L1 was constitutively expressed without simulation (0 IFN), however, after rIFN-γ stimulation at concentrations reached a plateau at 20–40 ng/mL. Data represent an average of three independent experiments.