Fig. 7.

PD-L1 gene suppression, by intravenous (I.V.) or intratracheal (I.T.) siRNA delivery, had no effect on cytokine/chemokine levels detected in bronchoalveolar lavage fluid (BALF). BALF was collected 24 h after hemorrhagic shock followed by cecal ligation and puncture (Hem-CLP) or sham operation. TNF-α, IL-6, IL-10, KC, MCP-1, and MIP-2 levels were increased after indirect acute lung injury (iALI) compared with sham mice. There were no changes in the cytokine/chemokine levels between intravenous and intratracheal delivery of PD-L1 siRNA in all iALI groups. Values are means ± SE; n = 6–10 mice per group, by one-way ANOVA and a Student–Newman–Keuls test.