Fig. 2.

A: lower respiratory tract recovery of FITC-dextran after tracheostomy and aerosol administration using the Scireq flexiVent rodent ventilator. During mechanical ventilation, various concentrations (20, 200, and 2,000 μg/mL) of aerosols were generated and administered over a fixed time period (120 s) to mice (n = 5–14 mice per group). B: recovered FITC-dextran correlated with the concentration administered (r = 0.969, P < 0.001) via the flexiVent, and recovery (%) was similar across all aerosol concentrations. C: FITC-dextran delivery/recovery was significantly reduced in younger mice, regardless of aerosol concentration. *P < 0.05, **P < 0.01, ***P < 0.001. D: size effect in C was also observed when delivery/recovery was correlated with body weight (BW) in adult mice.