Table 1.

Variables for administration of aerosols to mice via the inExpose system

Dose, μg	Aerosol Solution, μg/mL	Volume, μL	Nebulization Rate, μL/s	Exposure Time, s
5	200	25	2.22	11.25
5	100	50	2.22	22.5
5	50	100	2.22	45
5	25	200	2.22	90
5	20	250	2.22	112.5
5	10	500	2.22	225
5	5	1,000	2.22	450

Dosing of a fixed amount of aerosolized drug (μg) is a function of the fixed solution nebulization rate (2.22 μL/s) and 2 variables: duration of exposure (s) and volume of the solution (μL).