Fig. 4.

Sepsis causes oxidation of ryanodine receptor 2 (RyR2). A: representative Western blots of RyR2. B: blot of oxidized (oxy) RyR2 with loading control. C: quantification of oxidized RyR2. The means of the groups are different by ANOVA, *statistically significant difference from control by post hoc test. D: Western blots of sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA), phospholamban, and phosphorylated phospholamban, with loading control. E: quantification of SERCA and phosphorylated (P) phospholamban (PLN) in arbitrary units (AU). Phosphorylated phospholamban is normalized to total phospholamban. The results of ANOVA for these Western blots are not significantly different. WT, wild-type: CLP, cecal ligation and puncture surgery; KO, knockout.