Fig. 8.

A: representative immunohistochemistry sections for macrophage (CD68⁺) staining in frozen sections of left ventricle under baseline conditions. B: representative immunohistochemistry for T cell (CD3) staining in left ventricle sections under baseline conditions. C: quantitative CD68 analysis for WT and Tg mice. D: quantitative CD3⁺ analysis. Images were taken under ×20 objective and analyzed using ImageJ software. The number of cells were counted by a blinded observer and shown as number of positive cells per field. Data are presented as means ± SE. ***P < 0.005 vs. WT. n = 5–10 Mice per group. Scale bars are indicated in the lower right-hand corner of each panel. For statistical analysis, a 2-sample Wilcoxon test with the Fligner–Policello correction for unequal variances was used.