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Sequences of ISSR primers used to perform PCRs, number of bends amplified, polymorphic bands, and annealing temperature.
| Primer Name | DNA sequence (5’ 3’) | Total number of bands | Number of polymorphic bands | Annealing temperature (0С) |
|---|---|---|---|---|
| ISSR1 | AG (8)CTG | 6 | 6 | 48.2 |
| ISSR2 | AG (8)YT | 12 | 12 | 51.4 |
| ISSR3 | GA (8)YC | 14 | 13 | 53.9 |
| ISSR4 | CA (8)RC | 10 | 10 | 53.9 |
| ISSR5 | CA (7)RG | 13 | 12 | 49.0 |
| ISSR6 | CA (8)AA | 12 | 11 | 50.0 |
