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. 2020 Apr 29;21:18. doi: 10.1186/s12868-020-00565-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Successful establishing of HIE model in vivo. a The zea-longa scores in sham and HI groups. N = 20/group. b The comparison of TTC staining between sham and HI groups, and the white staining represented the ischemic area. N = 5/group. c The percentage of infarct area in sham and HI groups. d Tunel assay showed the apoptotic cells in right cortex. Red fluorescence represents the positive tunel staining, and blue is the nucleus staining with DAPI. N = 3/group. e Apoptosis rate in the right cortex, revealed by the tunel/DAPI. HI hypoxic-ischemic, h hour, TTC Triphenyte-trazoliumchloride, tunel terminal deoxynucleotidyl transferasedUtp nick end labeling staining. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01. Scale bar = 100 μm