Fig. 3.

The expression of COX5A after HI. a, b Representative images of immunohistochemical staining of COX5A in left- and right-cortex, Red arrows represent the COX5A positive cells, which were stained by the dark brown colour. N = 3/group. c, d Bar charts of the COX5A positive cells in left- and right- cortex. N = 3/group. e, f WB results of the COX5A protein expression in left- and right-cortex. N = 6/group. g, h qRT-PCR results of the COX5A mRNA expression in left- and right-cortex. N = 6/group. HI hypoxic-ischemic, COX5A cytochrome c oxidase subunit 5a, WB western blotting, qRT-PCR quantitative real-time polymerase chain reaction. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01. Scale bar = 200 μm